Wherever substances in complex matrices have to be analyzed HPTLC is the method of choice. It allows to easily confirm or reject the identity of raw materials. HPTLC is used in many laboratories worldwide for routine quality control.

Cosmetic products are often complex mixtures, difficult to analyze because of possible interferences by their different components. Therefore, the incorporation of active ingredients into the various formulations requires the development of suitable analytical methods for quality control. HPTLC has the capability for dealing with complex matrices. Examples for cosmetic applications can be found in our customer magazine CBS and the CCBS database. For example, in the CBS 118 (pp. 5–7) two typical applications are demonstrated. The first one describes a general method for detection and identification of UV filter in sun cream. The second one describes a method for the detection of phenolic markers specific to Edelweiss species in order to qualify different sources and grades of raw materials as well as glycerol-based cosmetic ingredients used for cosmeceutical products.

The use of standardized HPTLC methods allows you to clearly identify natural materials, regardless of the source, based on their chromatographic fingerprint. Additionally, HPTLC is a powerful tool to rapidly compare several samples in parallel, check for adulteration, monitor purity and stability, and quantify marker compounds.

Equip your lab with the CAMAG products that are most suitable for your specific needs and consult with us in the search for the solution to your analytical problems.

Matching CBS articles

  • Validated method for fast quantification of glycine in cosmetics
  • AMD analysis and determination of biocides in lens cleaning fluid
  • Quality control of cosmetic products by HPTLC
  • Quantification of xanthones in mangosteen fruit hull extracts
  • HPTLC bioautographic assay for tyrosinase inhibitors in plant extracts
  • Screening method to study the reactivity of cosmetic UV filters on skin proteins
  • Detection and determination of caffeine, taurine and arginine in shampoos
  • Quantification and side component analysis of the cosmetic active tiliroside using planar chromatography
  • Determination of aloe vera gel in cosmetics

Matching methods

  • A-104.1 – HPTLC fingerprint of Edelweiss plants and extracts used as ingredients in cosmeceuticals
  • A-103.1 – Detection of UV filters in cosmetic products by HPTLC and confirmation by HPTLC-MS
  • A-090.1 – HPTLC method for determination of ceramides from human skin